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KMID : 0380619840160020251
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Korean Journal of Food Science and Technology
1984 Volume.16 No. 2 p.251 ~ p.253
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A Simple and Modified Photometric Method for Measuring Lipase Activity
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Abstract
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A simple and modified method is developed to determine a lipase activity. A linear relationship exists between the color intensity and the free fatty acid liberated by enzyme action. The range of determination is from 0.05 to 1.5 moles of long chain fatty acid (oleic acid) and 0.2 to 2.0 μmoles of short chain fatty acid (caproic acid). The cumbersome procedure of the removal of the upper aqueous phase which was required in the previous copper soap extraction method was eliminated by the movement of solvent phase to upper phase in the respective biphasic system with a mixture solvent (chloroform: n-hexane:ethanol = 49:49:2) and copper reagent saturated with sodium chloride.
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